Home Research Project Details 2c - Fast 3D Calcium imaging for single action potential detection and temporal registration
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2c - Fast 3D Calcium imaging for single action potential detection and temporal registration

Detlev Schild and Fred Wolf

The goal of this project is to develop fast 3D calcium imaging techniques to record neuronal activity in the intact olfactory bulb with single action potential resolution. These techniques will be used to explore the impact of single neuron stimulation on distributed activity patterns in the intact network of the olfactory bulb and the potential to control distributed activity patterns in this network by minimal stimulation.

Ca2+ imaging is the most widely used method for the recoding of spatially distributed patterns of neuronal activity in vivo and in vitro (e.g. Zhang etal. 2007, Ohki et al. 2006). Although in principle single action potential detection can be achieved using this method, current use is largely restricted to qualitative assessment of response strength or to obtain indirect estimates of action potential count (e.g. Yaksi & Friedrich 2006).

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Fig. SP2c) (A) A fluorescence image of the Mitral cell layer of olfactory bulb stained with calcium indicator Fura-2. (B) Simultaneous recording of [Ca2+]-dependent fluorescence excited at 380nm, -F380 (top), and action potentials (bottom) of a Mitral cell. (C) Simultaneous recording of membrane potential in a Mitral cell (Cell1) during spontaneous [Ca2+] activity of its synchronous partner (Cell2). The voltage in cell 1 is locked to the [Ca2+] activity of cell 2. (D) Stimulating a patch-clamped neuron in depolarizing direction specifically enhances the [Ca2+] activity of its synchronous partner.

Belongs to Group(s):
Theoretical Neurophysics, Neurophysiology of the olfactory system

Is part of  Section 2 

Members working within this Project:
Schild, Detlev  
Wolf, Fred  

Selected Publication(s):

Dini, F, Martinelli, E, Paolesse, R, Filippini, D, Schild, D, Lundström, I, and Di Natale, C (2011).
Data processing for image-based chemical sensors: unsupervised region of interest selection and background noise compensation
Anal. Bioanal. Chem. 402(2):823-832.

Eifler, J, Martinelli, E, Santonico, M, Capuano, R, Schild, D, and Di Natale, C (2011).
Differential detection of potentially hazardous Fusarium species in wheat grains by an electronic nose
PLoS ONE 6(6):e21026.

Junek, S, Kludt, E, Wolf, F, and Schild, D (2010).
Olfactory coding with patterns of response latencies
Neuron 67(5):872-884.